Formerly International Journal of Basic and Applied Agricultural Research

Isolation, Screening and Characterization of Drought tolerant Plant Growth Promoting bacteria from Indian Himalayas

PRIYANKA KHATI, PANKAJ KUMAR MISHRA and LAKSHMI KANT
Pantnagar Journal of Research, Volume - 22, Issue - 2 ( May-August 2024)

Published: 2024-08-31

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Abstract


Drought is the one of the most hazardous abiotic stress resulting in huge losses to crop yield worldwide. Plants have evolved diverse ways to eliminate the harmful effect of different stresses. The acquisition of important plant growth promoting rhizobacteria (PGPR) in rhizosphere is the most effective mechanisms acquired by plants to ameliorate different stresses in soil. The objective of the research was to isolate, screen and characterized important PGP bacterial isolates along with potential of drought stress tolerance in Poly ethyle glycol (PEG)-6000 amended medium. About 232 bacterial isolates were isolated from different crop rhizosphere and subjected to drought stress tolerance assessment. Finally, 05 isolates were screened on the basis of critical drought tolerance and PGP characteristics (Zinc solubilization, Phosphate solubilization, siderophore production) under lab condition. The selected isolates were characterized morphologically, biochemically and through molecular methods using 16SrDNA region. The isolates will further act as repository of PGP characteristics along with water stress tolerance which can be tested and applied in field condition for plant stress amelioration.


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