Pantnagar Journal of Research

Abstract

The present study was undertaken on 50 unrelated Badri (hill cattle) of Kumaon region of Uttarakhand to explore polymorphism in intron 1 and 2 as well as exon 3 regions of leptin gene by PCR-RFLP technique and to find out the frequencies of different restriction fragment patterns for different regions. To reveal polymorphism in intron 1, a 340bp fragment within leptin gene was amplified and then digested with DraI restriction enzyme and study revealed three distinct types of genotypes or restriction patterns were observed. The genotype and gene frequencies were found to be 0.14, 0.40 and 0.46 for GG, GA and AA genotypes and 0.34 and 0.66 for G and A alleles, respectively. Restriction digestion of 422bp amplicons of intron 2 with Sau3AI revealed three genotypes. The genotype and gene frequency were found to be 0.56, 0.30 and 0.14 for AA, AB and BB genotypes and 0.71 and 0.29 for A and B alleles, respectively. The exon 3 fragment of 430bp size was digested with MspI and three distinct genotypes were observed. The genotype and gene frequency were found to be 0.26, 0.58 and 0.16 for CC, CT and TT genotypes and 0.55 and 0.45 for C and T alleles, respectively. The AA genotype in intron 1 was found to be relatively higher than GG genotype. The A allele of Sau3AI digestion within intron 2 was predominant and frequency of mutant allele T in exon 3 was found to be low