Formerly International Journal of Basic and Applied Agricultural Research

Thermal stability and catalytic efficiency of β-Glucosidase extracted from biogas slurry: Implications for biomass conversion

GAURAV SINGH RANA, A. K. VERMA and ASHUTOSH DUBEY
Pantnagar Journal of Research, Volume - 22, Issue - 3 ( September-December 2024)

Published: 2024-12-31

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Abstract


Biogas slurry (BGS), a nutrient-rich byproduct of anaerobic digestion, contains valuable enzymes like β glucosidase, essential for lignocellulosic biomass degradation. This study investigated the kinetic and thermodynamic properties of β-glucosidase extracted from BGS. The enzyme was purified through lyophilization and ammonium sulfate precipitation, resulting in an 8.3-fold increase in activity. Optimal enzyme activity was observed at 60°C and pH 7.0, with a Vmax of 4.207±0.07 mM/min, Km of 2.51±0.01 mM, and a catalytic efficiency (Kcat /Km) of 0.36±0.003 s/M. β-glucosidase exhibited high thermal and pH stability, retaining significant activity after prolonged exposure to high temperatures and varying pH levels. The activation energy (Ea) of 14.97±0.67 kJ/mol and a temperature coefficient (Q10) of 1.16±0.06, was indicating enzyme stability across a range of temperatures. These findings suggested that β-glucosidase from BGS could be a cost-effective and sustainable enzyme source for biofuel production from lignocellulosic biomass and could be suitable for large-scale waste valorization.


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