Pantnagar Journal of Research

Abstract

The current research aimed to investigate the changes in biochemical characteristics induced by nano-iron oxide and nano-aluminium oxide particles in Wistar rats during a 90-day repeated dose study. A total of 35 Wistar rats were randomly divided into two groups: control (with 20 animals) and test (with 15 animals). Throughout the duration of 90 days of the experiment, the control group received standard feed and purified reverse osmosis water. The treatment group was administered with a mixture of nano-iron oxide and nano-aluminium oxide in distilled water at a dose 15mg/kg body weight for nano-iron oxide and 3mg/kg body weight for nano-aluminium oxide, along with standard feed and RO water. Blood samples were collected from both G1 and G2 on 30th, 60th and 90th DPT for biochemical studies. There was significant increase in aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine and BUN whereas there was decrease in serum total protein, serum albumin, serum globulin and serum gamma globulin in treated group as compared to the control group. Based on the above findings, it concluded that combination of iron and aluminium nano particles at the rate of half of their NOAEL dose causes ill effects on the health status of Wistar Rats. It induces alteration in various biochemical parameters and thus causes health hazards.