Pantnagar Journal of Research

Abstract

Mastitis, a prevalent disease in high-yielding dairy animals, leads to substantial economic losses, further complicated by the rise of multidrug-resistant (MDR) bacteria, limiting conventional treatment options. This study aimed to evaluate the antibacterial efficacy of ethanolic clove (Syzygium aromaticum) extract against MDR pathogens isolated from mastitis-infected milk. Total 20 milk samples were collected, yielding MDR Staphylococcus aureus and Escherichia coli. The extract, prepared by maceration and confirmed to contain bioactive phytochemicals (flavonoids, tannins, phenols, etc.), was tested at varying concentrations using agar well diffusion assay. Results showed dose-dependent antibacterial activity, with the highest inhibition at 200 mg/ml. E. coli exhibited greater sensitivity (22.33±0.88 mm) than S. aureus (19.17±0.17 mm), and MIC values were 6.25 mg/ml for E. coli and 1.5625 mg/ml for S. aureus. The findings highlight clove extract's promising potential as a natural alternative for treating MDR mastitis pathogens.