Pantnagar Journal of Research

Abstract

Zinc is widely used in daily life, though it is considered comparatively safe; its pathological effects have not been documented. Limited investigations have been carried on effect of zinc nanoparticles on health of animals and man. Therefore, present study was designed to study the effect of zinc nano particles on haematological parameters of wistar rats. For this study, a total of 35 rats of six week age of both sexes approximately 50 gram weight were divided randomly into two groups of 20 rats in Group I (control) and 15 rats in group II(treated). In treated group rats were given nano zinc particles into stomach by oral gavaging and dose of nano zinc was selected based on NOAEL dose of nano zinc oxide 31.25 mg/kg body weight of rat for a period of 90 days. Blood was collected from 5 rats from each group at 0th (only from G1 group), 30th, 60th and 90th DPT for hematological studies. Hematological parameters like haemoglobin (Hb), packed cell volume (PCV), mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC), total leucocyte count (TLC), absolute lymphocyte count and lymphocyte count showed decrease in values compared to control. Significant decrease was observed in mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and lymphocyte count. There was increase in values of total erythrocyte count (TEC), monocyte count, eosinophil count, neutrophil count and absolute neutrophil count (ANC) as compared to control group. However, significant increase in total erythrocyte count (TEC), monocyte count and neutrophil count were observed.