Pantnagar Journal of Research

Abstract

Effluents from chemical industries are released abundantly into the environment many times without proper treatment. Such effluents find their way into the surrounding water bodies and thereby making water unfit for human and animal consumption. Limited investigations have been carried out on the impact of chemical industry effluents on health of animals and men. Therefore, present study was done to study the effect of chemical industry effluent on humoral immune response in mice. To study the effect of chemical industry effluent on humoral immune response, two-weeks-old 256 mice procured from Indian Veterinary Research Institute, Izatnagar, Bareilly were randomly divided into four equal groups of 64 mice each viz. control (group-1), R2B vaccine + effluent treated (group-2), effluent treated (group-3) and R2B vaccinated (group-4). The vaccine used was R2B strains given to group-2 and 4 @ 0.1 ml by intraperitoneal route. Humoral immune response was assessed by Lymphocyte Stimulation Test using LPS and by performing ELISA at 15th, 30th,45th,60th,75th,90th,105th and 120th day post treatment DPT. The mean OD and antibody titer was significantly lower in group 2 and group 3 mice on 90, 105 and 120 days of observation in comparison to groups 1 and 4 mice. These findings clearly suggest that the metal exposed animals might have a decreased ability to develop humoral immune responses.