Pantnagar Journal of Research

Abstract

The study was conducted to isolate bacterial microflora from tracheo-bronchial aspirates (TBA) of healthy and pneumonic crossbred adult cattle. A total of fifty cross bred adult cattle diagnosed for pneumonia were selected. Ten apparently healthy cattle were included as healthy control animals. TBA samples were collected aseptically by percutaneous method. TBA sediment was inoculated on Blood agar and MacConkey’s lactose agar. Bacterial isolates were identified and characterized on the basis of cultural examination and biochemical tests. TBA from six healthy animals and 44 pneumonic cattle were positive for bacterial growth. Out of 85 isolates obtained from pneumonic cows, Staphylococcus spp. other than S. aureus (25.9 %) were the most prevalent followed by Pasturella multocida (12.9 %), Bacillus spp. (11.8 %), Pseudomonas spp. (10.6 %), Staphylococcus aureus (10.6 %), E. coli (9.4 %), Klebsiella spp. (8.2 %), Proteus spp. (7.1 %) and Streptococcus spp. (3.5%)